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Protein information

Protein name SH3-domain kinase binding protein 1 
Synonyms Cbl-interacting protein of 85 kDa
Human Src-family kinase binding protein 1
HSB-1
CD2 binding protein 3
CD2BP3
Gene name SH3KBP1 , CIN85 .
Source Homo sapiens (Human)
Expression system HEK293T
Full length  665
Precursor  no
Mature form length  665
Molecular weight (Da)  73126
Swiss-Prot Sequence Version  May 23, 2003 (Sequence version 2)

Cross-references

Swiss-Prot/TrEMBL SH3K1_HUMAN
PDB 2BZ8 .

Ubiquitylation features

Ubiquitylation site  
 

Ubiquitylation type Mono-ubiqutylation
Features CIN85 and its homologue CMS are monoubiquitinated by Cbl/Cbl-b after epidermal growth factor (EGF) stimulation. Ubiquitin acceptor site present in the carboxyl terminus of CIN85. Extensive studies indicated that Cbl-directed monoubiquitination of CIN85 is not specific for a particular lysine residue but rather utilizes various lysine residues in the context of the intact carboxyl terminus of CIN85.

Ubiquitylation/deubiquitylation machinery

E2 Not determined
E3 CBL
E4/AP Not determined
DUB Not determined
Comments Cbl-b and monoubiquitinated CIN85 are found in the complex with polyubiquitinated EGF receptor tyrosine kinases during prolonged EGF stimulation and are degraded together in the lysosome.

References

[1]Haglund K, Shimokawa N, Szymkiewicz I, Dikic I
Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand-induced degradation of EGF receptors. Proc.Natl.Acad.Sci U.S.A 2002, 99:12191-12196.
[12218189]
 

Ubiquitylation type Multiple ubiquitylation
Features Overexpression of Nedd4 induced multiubiquitination of both CIN85 and CMS independently of EGF stimulation [1]. All three isoforms of Ruk (CIN85 = L, M and S) are multiubiquitinated [2].

Ubiquitylation/deubiquitylation machinery

E2 Not determined
E3 Nedd4 [1]; CBL [2]
E4/AP Not determined
DUB Not determined
Comments The use of proteosomal and lysosomal inhibitors clearly indicated that ubiquitination of CIN85 (Ruk L isoform) does not lead to its degradation [2].

References

[1]Haglund K, Shimokawa N, Szymkiewicz I, Dikic I
Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand-induced degradation of EGF receptors. Proc.Natl.Acad.Sci U.S.A 2002, 99:12191-12196.
[12218189]
[2]Verdier F, Valovka T, Zhyvoloup A, Drobot LB, Buchman V, Waterfield M, Gout I:
Ruk is ubiquitinated but not degraded by the proteasome. Eur.J Biochem 2002, 269:3402-3408.
[12135478]

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