UbiProt Database - proteins_show

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Protein information

Protein name Ubiquitin-protein ligase E3 Mdm2 
Synonyms EC 6.3.2.-
p53-binding protein Mdm2
Oncoprotein Mdm2
Double minute 2 protein
Hdm2
Gene name MDM2 .
Source Homo sapiens (Human)
Expression system Escherichia coli BL-21
Full length  491
Precursor  no
Mature form length  491
Molecular weight (Da)  55233
Swiss-Prot Sequence Version  April 1, 1993 (Sequence version 1)

Cross-references

Swiss-Prot/TrEMBL MDM2_HUMAN
PDB 1RV1 , 1T4E , 1T4F , 1YCR , 1Z1M , 2C6A , 2C6B , 2F1Y .

Ubiquitylation features

Ubiquitylation site  
 

Ubiquitylation type Not Determined
Features GST-Mdm2 was immobilized on glutathione-Sepharose beads (GS) followed by a ubiquitination reaction using recombinant 32 P-labeled Ub in the presence of E1 and E2 (UbcH5B), both of which were also expressed as recombinant proteins in E. coli.

Ubiquitylation/deubiquitylation machinery

E2 UbcH5B
E3 Not determined
E4/AP Not determined
DUB Not determined
Comments Autoubiquitylation; in vitro

References

[1]Fang S, Jensen JP, Ludwig RL, Vousden KH, Weissman AM
Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53. J Biol.Chem. 2000, 275:8945-8951.
[10722742]

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