UbiProt Database - proteins_show

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Protein information

Protein name Proteinase-activated receptor 2 
Synonyms PAR-2
Thrombin receptor-like 1
Coagulation factor II receptor-like 1
G-protein coupled receptor 11
Gene name F2RL1 (GPR11, PAR2) .
Source Homo sapiens (Human)
Expression system Rat (KNRK) and human (HEK293) kidney epithelial cells
Full length  397
Precursor  yes
Mature form length  361
Molecular weight (Da)  44126
Swiss-Prot Sequence Version  October 1, 1996 (Sequence version 1)
Comments  PAR2 is a G-protein coupled receptor. Activation occurs by cleavage of N-terminal propeptide.

Cross-references

Swiss-Prot/TrEMBL PAR2_HUMAN
PDB  

Ubiquitylation features

Ubiquitylation site Not determined
 

Ubiquitylation type Pluri-ubiquitylation
Features Ubiquitylated PAR2 is not recognized with an antibody that only recognizes polyubiquitylated proteins, indicating that PAR2 is mono-ubiquitylated. Large molecular weight of ubiquitylated PAR2 compared with the unstimulated receptor suggests that PAR2 undergoes mono-ubiquitylation at multiple lysine residues (i.e. multimono-ubiquitylation or pluri-ubiquitylation).

Ubiquitylation/deubiquitylation machinery

E2 Not determined
E3 c-Cbl
E4/AP Not determined
DUB Not determined
Comments Activation of wild type PAR2 stimulates tyrosine phosphorylation of c-Cbl ligase and promotes its interaction with PAR2 at the plasma membrane and in endosomes in an Src-dependent manner. c-Cbl mediates ubiquitylation and lysosomal degradation of PAR2 to terminate signaling by this and perhaps other GPCRs.

References

[1]Jacob C, Cottrell GS, Gehringer D, Schmidlin F, Grady EF, Bunnett NW.
c-Cbl mediates ubiquitination, degradation, and down-regulation of human protease-activated receptor 2. J Biol Chem. 280(16):16076-87 (2005).
[15708858]

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