UbiProt Database - proteins_show

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Protein information

Protein name General amino-acid permease GAP1 
Gene name GAP1 , YKR039W .
Source Saccharomyces cerevisiae (Baker's yeast)
Expression system Saccharomyces cerevisiae (Baker's yeast)
Full length  602
Precursor  no
Mature form length  602
Molecular weight (Da)  65656
Swiss-Prot Sequence Version  June 1, 1994 (Sequence version 2)



Ubiquitylation features

Ubiquitylation site K76

Ubiquitylation type Not Determined
Features Not determined

Ubiquitylation/deubiquitylation machinery

E2 Not determined
E3 Npi1/Rsp5 [3]
E4/AP Not determined
DUB Not determined
Comments Not determined


[1]Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
A proteomics approach to understanding protein ubiquitination. Nat.Biotechnol. 2003, 21:921-926.
[2]Hitchcock AL, Auld K, Gygi SP, Silver PA.
A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery. Proc.Natl.Acad.Sci U.S.A 2003, 100:12735-12740.
[3]Springael JY, Andre B
Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae. Mol Biol.Cell 1998, 9:1253-1263.

Ubiquitylation type Lys63-linked multi-ubiquitin chain
Features Not determined

Ubiquitylation/deubiquitylation machinery

E3 Npi1p/Rsp5p [1]
E4/AP Not Determined
DUB Npi2p/Doa4p/Ubp4p/Ssv7p [1]
Comments Not determined


[1]Springael JY, Galan JM, Haguenauer-Tsapis R, Andre B
NH4+-induced down-regulation of the Saccharomyces cerevisiae Gap1p permease involves its ubiquitination with lysine-63-linked chains. J Cell Sci 1999, 112 ( Pt 9):1375-1383.
[2]Helliwell SB, Losko S, Kaiser CA.
Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease. J.Cell Biol. 2001, 153:649-662.
[3]Soetens O, De Craene JO, Andre B.
Ubiquitin is required for sorting to the vacuole of the yeast general amino acid permease, Gap1. J.Biol.Chem. 2001, 276:43949-43957.
[4]Nikko E, Marini AM, Andre B.
Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway. J.Biol.Chem. 2003, 278:50732-50743.


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