UbiProt Database - proteins_show

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Protein information

Protein name PCNA_proliferating cell nuclear antigen 
Synonyms PCNA
Cyclin
Gene name PCNA .
Source Homo sapiens (Human)
Expression system HEK293
Full length  261
Precursor  no
Mature form length  261
Molecular weight (Da)  28769
Swiss-Prot Sequence Version  October 1, 1989 (Sequence version 1)

Cross-references

Swiss-Prot/TrEMBL PCNA_HUMAN
PDB 1AXC , 1U76 , 1U7B , 1UL1 , 1VYJ , 1VYM , 1W60 , 2ZVK , 2ZVL , 2ZVM .

Ubiquitylation features

Ubiquitylation site K164
 

Ubiquitylation type Mono-ubiqutylation
Features PCNA becomes monoubiquitinated following UV irradiation of the cells.

Ubiquitylation/deubiquitylation machinery

E2 Not Determined
E3 hRad18
E4/AP Not Determined
DUB Not Determined
Comments Monoubiquitinated PCNA but not unmodified PCNA specifically interacts with DNA polymerase eta

References

[1]Kannouche PL, Wing J, Lehmann AR
Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage. Mol Cell 2004, 14:491-500.
[15149598]
[2]Laub M, Rumpf H, Jennissen HP:
Analysis of substrate specificity of ubiquitin-calmodulin ligase: biochemical versus molecular biology approach. Biological Chemistry 2000, 381:S255.
[0]
 

Ubiquitylation type Lys63-linked multi-ubiquitin chain
Features Mono-ubiquitylation induces non-canonical poly-ubiquitination through Lys-63 linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases RNF8 and SHPRH, which are required for DNA repair.

Ubiquitylation/deubiquitylation machinery

E2 UBE2N-UBE2V2 complex
E3 SHPRH
E4/AP Not Determined
DUB Not Determined
Comments Not Determined

References

[1]Unk I, Hajd? I, F?tyol K, Szak?l B, Blasty?k A, Bermudez V, Hurwitz J, Prakash L, Prakash S, Haracska L
Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen. Proc Natl Acad Sci U S A 2006, 103:18107-12
[17108083]
 

Ubiquitylation type Not Determined
Features  

Ubiquitylation/deubiquitylation machinery

E2 Not Determined
E3 Not Determined
E4/AP Not Determined
DUB Not Determined
Comments

References

[1]Matsumoto M, Hatakeyama S, Oyamada K, Oda Y, Nishimura T, Nakayama KI
Large-scale analysis of the human ubiquitin-related proteome. Proteomics 2005, 5:4145-51.
[16196087]

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