UbiProt Database - proteins_show

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Protein information

Protein name 3-hydroxy-3-methylglutaryl-coenzyme A reductase 
Synonyms EC 1.1.1.34
HMG-CoA reductase
HMGR
Gene name HMGCR .
Source Homo sapiens (Human)
Expression system HEK293, LP-90
Full length  888
Precursor  no
Mature form length  888
Molecular weight (Da)  97476
Swiss-Prot Sequence Version  November 1, 1986 (Sequence version 1)
Comments  HMGR is an enzyme of sterol biosynthetic pathway. Transmembrane ER-associated glycoprotein, catalitically active in homodimeric form.

Cross-references

Swiss-Prot/TrEMBL HMDH_HUMAN
PDB 1DQA , 1DQ9 , 1DQ8 , 1HWL , 1HWK , 1HWI , 1HW9 , 1HW8 , 1HWJ .

Ubiquitylation features

Ubiquitylation site K89, K248, N-terminus
 

Ubiquitylation type Multiple ubiquitylation
Features Multiubiquitylation of HMGR leads to its proteasomal degradation. Ubiquitylation may affect N-terminus in addition to 2 identified sites [1].

Ubiquitylation/deubiquitylation machinery

E2 Not determined
E3 HRD1
E4/AP Not determined
DUB Not determined
Comments HRD1 is a non-glycosylated ER protein with a cytosolic RING-H2 finger domain. In vitro it shows ability to form Lys48-polymerized multiubiquitin chains in the presence of UBC7. Human HRD1 is involved in the basal degradation of HMGR but not in the sterol-regulated degradation. HRD1 is also involved in the elimination of model ER-associated degradation substrates TCR-alpha and CD3-delta [2].

References

[1]Doolman R, Leichner GS, Avner R, Roitelman J.
Ubiquitin is conjugated by membrane ubiquitin ligase to three sites, including the N terminus, in transmembrane region of mammalian 3-hydroxy-3-methylglutaryl coenzyme A reductase: implications for sterol-regulated enzyme degradation. J Biol Chem. 279(37):38184-93 (2004).
[15247208]
[2]Kikkert M, Doolman R, Dai M, Avner R, Hassink G, van Voorden S, Thanedar S, Roitelman J, Chau V, Wiertz E.
Human HRD1 is an E3 ubiquitin ligase involved in degradation of proteins from the endoplasmic reticulum. J Biol Chem. 279(5):3525-34 (2004).
[14593114]

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