UbiProt Database - proteins_show

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Protein information

Protein name General control protein GCN4 
Synonyms Amino acid biosynthesis regulatory protein
Gene name GCN4 , AAS3 , ARG9 , YEL009C .
Source Saccharomyces cerevisiae (Baker's yeast)
Expression system Saccharomyces cerevisiae (Baker's yeast)
Full length  281
Precursor  no
Mature form length  281
Molecular weight (Da)  31310
Swiss-Prot Sequence Version  July 21, 1986 (Sequence version 1)


PDB 1CE9 , 1DGC , 1GCL , 1GCM , 1IHQ , 1KQL , 1LLM , 1PIQ , 1SWI , 1TMZ , 1YSA , 1ZII , 1ZIJ , 1ZIK , 1ZIL , 1ZIM , 1ZTA , 2DGC , 2ZTA .

Ubiquitylation features

Ubiquitylation site ND

Ubiquitylation type Not Determined
Features This ubiquitylated protein was isolated uniquely from cells lacking Rpn10 (poly-ubiquitin receptor of 26S proteasome), suggesting that it usually undergoes rapid protesomal degradation (see Ref. 2).

Ubiquitylation/deubiquitylation machinery

E2 UBC3 / CDC34 (see Ref. 1), UBC2 / RAD6 (see Ref. 4)
E3 SCF(CDC4) (see Ref. 1), SCF (CDC4,cul1) (see Ref. 3)
E4/AP Not determined
DUB Not determined
Comments Not determined


[1]Meimoun A, Holtzman T, Weissman Z, McBride HJ, Stillman DJ, Fink GR, Kornitzer D
Degradation of the transcription factor Gcn4 requires the kinase Pho85 and the SCF(CDC4) ubiquitin-ligase complex. Mol Biol.Cell 2000, 11:915-927.
[2]Mayor T, Russell L. J., Graumann J., Smith G. T., Deshaies R. J.:
Analysis of poly-ubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets. Mol Cell Proteomics 2005 4(6):741-751
[3]Petroski,M.D.; Deshaies,R.J.:
Function and regulation of cullin-RING ubiquitin ligases; Nat.Rev.Mol Cell Biol., 2005, 6, 1, 9-20
[4]Kornitzer D, Raboy B, Kulka RG, Fink GR.
Regulated degradation of the transcription factor Gcn4. EMBO J. 1994 Dec 15;13(24):6021-30.


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